Higher Level Expression of Enzyme

Day 1 | Day 2 | Download Brochure | Speaker Bios 


Enzymes are an important staple in the biopharmaceutical, agricultural and chemical fields. However, expression of these workhorse proteins comes with a number of problems as well. Bacterial hosts can produce large quantities of these proteins, but struggle when tasked with expression of very large proteins or proteins that require post-translational modifications. Fungal expression systems have the potential to produce toxins during the expression cycle, which can make their product unusable. Both types of hosts must undergo the extreme physiological impacts of high-level expression of the genes needed to create over-production.

This inaugural conference will address the methods and technologies to create high-level expression of enzymes for therapeutics, food sciences and bioenergy in microbes and other host systems.


THURSDAY, MAY 8


RECOMMENDED PRE-CONFERENCE SHORT COURSES*

Antibody Humanization via One Hot Homology Model (Hands-On) Workshop - View Short Courses Page
In silico Immunogenicity Predictions (Hands-On) Workshop - View Short Courses Page 

*Separate registration required


The Science for Success 

1:30 pm Chairperson's Opening Remarks

Markku Saloheimo, Ph.D., VTT Technical Research Centre of Finland

1:40 Class A β-Lactamases as Versatile Scaffolds to Create Hybrid Enzymes: Applications from Basic Research to Medicine

Marylene VandevenneMarylene Vandevenne, Ph.D., Postdoctoral Research Associate, Mackay Laboratory, The School of Molecular Bioscience, The University of Sidney - Australia

Designing hybrid proteins is a major aspect of protein engineering and covers a very wide range of applications from basic research to medical applications. This review focuses on the use of class A β -lactamases as versatile scaffolds to design hybrid enzymes (referred to as β -lactamase hybrid proteins, BHPs) in which an exogenous peptide, protein or fragment thereof is inserted at various permissive positions. We discuss how BHPs can be specifically designed to create bifunctional proteins, to produce and to characterize proteins that are otherwise difficult to express, to determine the epitope of specific antibodies, to generate antibodies against nonimmunogenic epitopes, and to better understand the structure/function relationship of proteins.

2:10 The Cargo and the Transport System: Secreted Proteins and Protein Secretion in Trichoderma reesei (Hypocrea jecorina)

Markku Saloheimo, Ph.D., VTT Technical Research Centre of Finland

The filamentous fungus Trichoderma reesei is an enzyme production host utilised widely by the industry. Its efficiency in production is not surpassed by any other production platform, as secreted protein yields of over 100 g/l from the system have been reported. The talk will elucidate the special features of T. reesei as a production host, the challenges connected to recombinant protein production and our efforts to tackle these challenges.

2:40 Sponsored Presentation (Opportunity Available)

2:55 Sponsored Presentation (Opportunity Available)

3:10 Refreshment Break in the Exhibit Hall with Poster Viewing

4:00 Problem Solving Breakout Discussions

Cell-Free Technologies for Supporting Enzyme Expression and Function

Matthew ColemanModerator: Matthew Coleman, Ph.D., Professor, Radiation Oncology, University of California at Davis

  • Capabilities
  • Limitations
  • Making the cell-free decision

Determining Best Expression Systems for Different Enzymes

Rima MenassaModerator: Rima Menassa, Ph.D., Research Scientist, Adjunct Professor, Agriculture and Agri-Food Canada

  • Enzyme characteristics, post-translational modifictions
  • To secrete or not to secrete? Subcellular effects on enzyme accumulation and activity
  • Cost of production
 

Engineering Proteins with High Affinity While Maintaining High Specificity

Marylene VandevenneModerator: Marylene Vandevenne, Ph.D., Postdoctoral Researcher, Mackay Lab, University of Sydney - Australia

 

5:00 Close of Day


RECOMMENDED THURSDAY DINNER SHORT COURSE*

Protein Aggregation: Mechanism, Characterization and Immunogenic Consequences - View Detailed Agenda 

*Separate registration required


Day 1 | Day 2 | Download Brochure | Speaker Bios