SC13 DETAILED AGENDA
Tuesday, May 1, 2012
6:00-9:00PM
Kevin Mattison, Ph.D., Principal Scientist, Bioanalytics, Malvern Instruments
Ulf Nobbmann, Ph.D., SEC & Light Scattering Specialist, Malvern Instruments
Jean-Luc Brousseau, Ph.D., SEC & Light Scattering Specialist, Malvern Instruments
Since its commercialization circa 30 years back, light scattering instrumentation has occupied a position of increasing popularity within the area of protein aggregate detection and formulation characterization, due in large part to the non-invasiveness of the technique, the minimal sample volume & concentration requirements, and the quickness of data collection. While modern instrument design and software have removed much of the mystic traditionally associated with the technique, data interpretation is still a common source of frustration for new light scattering users. This workshop covers the basic theory behind static, dynamic, and electrophoretic light scattering, with a focus on the do’s & don’ts when it comes to data interpretation. Key Discussion Points:
- How is the mass distribution determined in DLS & what is the accuracy?
- Can light scattering be used for particle counting?
- How do I convert zeta potential to surface charge?
- What's the relationship between the 2nd virial coefficient and the DLS interaction parameter?
- How does light scattering compare to other techniques such as SEC & AUC?
Full Agenda:
6:00 Welcome and Introduction to Moderator and Panelists
6:15 Quick poll and interview of attendees to gauge their backgrounds, level of experience, purposes for attending and desired takeaways
6:30 Workshop Part 1, Formal Presentations
7:15 Workshop Part 2, Interactive Q&A w/ Moderator and Panel
7:45 Workshop Part 3, Informal discussions, examples from attendees
8:30 Question and Answer Period
9:00 End of Short Course
Speaker Bios:
Kevin Mattison, Ph.D., Principal Scientist, Bioanalytics, Malvern Instruments
Kevin completed his doctorate in biological chemistry at Purdue University, where he studied the effects of polyelectrolyte additives on the stability and activity of transport proteins and enzymes. From there he joined Protein Solutions as the Applications Development and Technical Support Manager, and was instrumental in helping to drive the adoption of sub-micron light scattering techniques from esoteric technologies into main stream laboratory tools. In 2002 Kevin joined Malvern Instruments, where he served as Applications Manager, Product Manager, and Director of Customer Support, prior to assuming his current position as Principal Scientist – Bioanalytics in the Strategic Technology Development Group.
Ulf Nobbmann, Ph.D., SEC & Light Scattering Specialist, Malvern Instruments
Ulf received his PhD in colloidal physics from Oklahoma State University, where he investigated the scattering of light from micron & sub-micron sized particle systems under both dilute and concentrated conditions. Upon graduation, he joined Protein Solutions, where he turned his attention to the application of colloidal principles and characterization techniques to biological molecules and nano particles. Ulf has written book chapters and scientific articles covering relevant aspects and applications in industry and biotechnology. He joined Malvern Instruments in 2003, where he served as Product Manager for their multi-angle light scattering systems prior to assuming his current role as Technical/Scientific Advisor for Light Scattering & GPC/SEC Technologies.
Jean-Luc Brousseau, Ph.D., SEC & Light Scattering Specialist, Malvern Instruments
Dr. Jean-Luc Brousseau received his Ph.D. in Biophysics from the Universite du Quebec a Trois-Rivieres in 1999. His Ph.D. research was conducted at the University of Miami on macromolecules and sol-gels. His Post Doctoral research at Tulane University was on light scattering of polymer and novel characterization of polymerization reaction. At Tulane Dr. Brousseau developed an online characterization technique for measuring polymerization reaction kinetics. He then joined Brookhaven Instruments as a development specialist designing and introducing many instruments. He worked as a senior scientist at Brookhaven for 5 years on light scattering of proteins and polymers. Dr. Brousseau joined Malvern Instrument in 2007 where he was a Development Specialist for the flow-mode absolute size exclusion chromatography (ASEC) for the Zetasizer Nano light scattering instrument. He is now in the separation division working with the Viscotek line of instruments.
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