CHI’s 10th annual Difficult to Express Proteins conference will present the most up-to-date strategies and technologies for successfully expressing and purifying the proteins that keep researchers up at night. Whether because of folding problems, toxicity to hosts, purification issues or any of a host of other problems, these proteins resist standard expression and purification methods.


Development of an Improved Mammalian Overexpression Method for Human CD62L

Peter D. Sun, Ph.D., Structural Immunology Section, Lab of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health

Overexpression of Pseudomonas aeruginosa LpxC with Its Inhibitors in an acrB-Deficient Escherichia coli Strain

Ning Gao, Associate Principal Scientist, Discovery Sciences Innovative Medicine Unit, AstraZeneca R&D


Recombinant Expression, Purification, and Biophysical Characterization of the Transmembrane and Membrane Proximal Domains of HIV-1 gp41

Tsafrir Mor, Ph.D., Associate Professor, The Biodesign Institute, Infectious Diseases and Vaccinology, Arizona State University

G Protein-Coupled Receptor Expression and Purification

Sotirios Koutsopoulos, Ph.D., Research Scientist, Center for Biomedical Engineering, Massachusetts Institute of Technology

Small Expression Tags Enhance Bacterial Expression of the First Three Transmembrane Segments of the Apelin Receptor

Aditya Pandey, Ph.D., Department of Biochemistry & Molecular Biology, Dalhousie University

New Tools for Protein Solubility

David Mead, Ph.D., Founder & CSO, Lucigen Corp


Expression of Difficult-to-Express Proteins via Novel in silico Software Coupled with Multi-Modal Expression Screen

Prabuddha K. Kundu, Ph.D., Co-founder & Executive Director, Premas Biotech Pvt Ltd


Optimized E. coli Expression Strain LOBSTR Eliminates Common Contaminants from His-Tag Purification

Thomas U. Schwartz, Ph.D., Principal Investigator, Department of Biology, Massachusetts Institute of Technology

Expression, Purification, and Micelle Reconstitution of Antimicrobial Piscidin 1 and Piscidin 3 for NMR Studies

Wen Chen, Ph.D., Department of Biological Chemistry & Molecular Pharmacology, Harvard Medical School


TAPBOOST Technology: Enhanced Production for Hard-to-Produce Proteins

Akinori Hishiya, Ph.D., Director, Biology, Boston Strategics Corporation

Enhanced Transient Recombinant Protein Production in CHO Cells through the Co-Transfection of the Product Gene with Bcl-xL

Matthew Zustiak, Ph.D., Researcher, Gallus Biopharmaceuticals

It's Time to Regulate: Coping with Product-Induced Nongenetic Clonal Instability in CHO Cell Lines via Regulated Protein Expression

Shahram Misaghi, Ph.D., Scientist, Early Stage Cell Culture, Genentech, Inc.

For more details on the conference, please contact:
Elizabeth J. Lamb
Senior Conference Director
Cambridge Healthtech Institute
T: 781-247-6259

For partnering and sponsorship information, please contact:
Jason Gerardi (Companies A-K)
Manager, Business Development
Cambridge Healthtech Institute
T: 781-972-5452

Carol Dinerstein (Companies L-Z)
Director, Business Development
Cambridge Healthtech Institute
T: 781.972.5471