Cambridge Healthtech Institute’s 20th Annual
Difficult-to-Express Proteins
May 12-13, 2025
The structural and functional study of proteins, as well as the development of targeted biotherapeutics, depends critically on the ability to express, produce, and purify recombinant proteins. However, some proteins present significant challenges in these processes due to factors such as complex folding, host toxicity, and purification difficulties. The 20th Annual Difficult-to-Express Proteins conference, organized by Cambridge Healthtech Institute, explores these challenges in depth. This conference offers a platform for researchers to share innovative approaches and advanced technologies that have proven effective in overcoming the hurdles associated with producing high yields of these difficult-to-express proteins (DTEPs).
Coverage will include, but is not limited to:
- High-yield production (strategies for boosting output of difficult-to-express proteins)
- Membrane proteins (approaches to express and purify GPCRs, ion channels, and other complex proteins)
- Enzymes (challenges with caspases, proteases, and kinases)
- Disulfide-rich proteins (overcoming folding and stability issues)
- Tagged/fused proteins (leveraging tags and fusion strategies to simplify purification)
- Designer proteins (expressing novel engineering proteins with custom functions)
- Misfolding and aggregation (tackling folding problems and preventing aggregation)
- Expression location (optimizing expression in cytoplasm, periplasm, or extracellular space)
- Purification (advances in protein purification methods)
- Emerging tools & technologies (emerging innovations for enhancing expression and production)
- Case studies (real-world applications and success stories)
The deadline for priority consideration is October 11, 2024.
All proposals are subject to review by session chairpersons and/or the Scientific Advisory Committee to ensure the overall quality of the conference program. Additionally, as per Cambridge Healthtech Institute’s policy, a select number of vendors and consultants who provide products and services will be offered opportunities for podium presentation slots based on a variety of Corporate Sponsorships.
Opportunities for Participation: