Cambridge Healthtech Institute’s 16th Annual

Difficult-to-Express Proteins

May 11-12, 2021


What makes a protein ‘difficult’ to produce? CHI’s 16th Annual Difficult-to-Express Proteins conference examines the challenges encountered by researchers when striving for high-yield production of “difficult-to-express” proteins (DTEPs), and the strategies and technologies that have proven successful in overcoming those challenges. The Difficult-to-Express Proteins conference provides the latest developments in improving yield for DTEPs through case studies and breakthrough data.


Coverage will include, but is not limited to:


  • Taming difficult-to-express proteins
  • High-yield production of “difficult-to-express” proteins
  • Membrane proteins (GPCRs, ion channels, etc.)
  • Caspases
  • Proteases
  • Kinases
  • Genetic engineering
  • Gene expression
  • Translation
  • Secretion
  • Re-engineering
  • Physical properties
  • Amino acid sequence
  • Disulfide-rich proteins
  • Disulfide bond stability
  • Overcoming misfolding
  • Aggregation
  • Cryo-EM
  • Crystallization
  • Emerging tools & technologies
  • Achieving soluble protein
  • Tagged or fused proteins
  • To express in the cytoplasm, periplasm or extracellular in the medium?


If you would like to submit a proposal to give a presentation at this meeting, please click here.


All proposals are subject to review by session chairpersons and/or the Scientific Advisory Committee to ensure the overall quality of the conference program. Additionally, as per Cambridge Healthtech Institute’s policy, a select number of vendors and consultants who provide products and services will be offered opportunities for podium presentation slots based on a variety of Corporate Sponsorships.


For more details on the conference, please contact:
Mary Ann Brown

Executive Director, Conferences

Phone: 781-972-5497


For partnering and sponsorship information, please contact:

Companies A-K

Jason Gerardi

Senior Manager, Business Development

Phone: 781-972-5452


Companies L-Z

Ashley Parsons

Manager, Business Development

Phone: 781-972-1340